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已发表论文
使用脂质体对长春新碱 (Vincristine) 硫酸盐共轭的金纳米粒子的递送： 提高抗肿瘤疗效的光响应纳米载体
Authors Liu Y, He M, Niu MM, Zhao YQ, Zhu YZ, Li ZH, Feng NP
Published Date April 2015 Volume 2015:10 Pages 3081—3095
DOI http://dx.doi.org/10.2147/IJN.S79550
Received 18 December 2014, Accepted 31 January 2015, Published 22 April 2015
Abstract: Rapid drug release at the specific site of action is still a challenge
for antitumor therapy. Development of stimuli-responsive hybrid nanocarriers
provides a promising strategy to enhance therapeutic effects by combining the
unique features of each component. The present study explored the use of
drug–gold nanoparticle conjugates incorporated into liposomes to enhance
antitumor efficiency. A model drug, vincristine sulfate, was physically
conjugated with gold nanoparticles and verified by UV-visible and fourier
transform infrared spectroscopy, and differential scanning calorimetry. The
conjugates were incorporated into liposomes by film dispersion to yield
nanoparticles (113.4 nm) with light-responsive release properties, as shown by
in vitro release studies. Intracellular uptake and distribution was studied in
HeLa cells using transmission electron microscopy and confocal laser scanning
microscopy. This demonstrated liposome internalization and localization in
endosomal–lysosomal vesicles. Fluorescence intensity increased in cells exposed
to UV light, indicating that this stimulated intracellular drug release; this
finding was confirmed by quantitative analyses using flow cytometry. Antitumor
efficacy was evaluated in HeLa cells, both in culture and in implants in vivo
in nude mice. HeLa cell viability assays showed that light exposure enhanced
liposome cytotoxicity and induction of apoptosis. Furthermore, treatment with
the prepared liposomes coupled with UV light exposure produced greater
antitumor effects in nude mice and reduced side effects, as compared with free
vincristine sulfate.
Keywords: vincristine sulfate, vincristine sulfate–gold nanoparticle conjugates, liposomes, antitumor efficiency, light triggered drug release
Keywords: vincristine sulfate, vincristine sulfate–gold nanoparticle conjugates, liposomes, antitumor efficiency, light triggered drug release
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