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已发表论文

一种有效给药系统用于新城疫病毒 DNA 疫苗的脱乙酰壳多糖包衣聚（乳酸–共轭-乙醇酸）纳米颗粒

 

Authors Zhao K, Zhang Y, Zhang X, Shi C, Wang X, Wang X, Jin Z, Cui S

Published Date September 2014 Volume 2014:9(1) Pages 4609—4619

DOI http://dx.doi.org/10.2147/IJN.S70633

Received 4 July 2014, Accepted 24 July 2014, Published 30 September 2014

Approved for publication by Professor Thomas J. Webster

Abstract: We determined the efficacy and safety of chitosan (CS)-coated poly(lactic-co-glycolic) acid (PLGA) nanoparticles (NPs) as a delivery system for a vaccine to protect chickens against Newcastle disease virus (NDV). The newly constructed vaccine contained DNA (the F gene) of NDV. The Newcastle disease virus (NDV) F gene deoxyribonucleic acid (DNA) plasmid (pFDNA)-CS/PLGA-NPs were spherical (diameter =699.1±5.21 nm [mean ± ­standard deviation]) and smooth, with an encapsulation efficiency of 98.1% and a Zeta potential of +6.35 mV. An in vitro release assay indicated that CS controlled the burst release of plasmid DNA, such that up to 67.4% of the entire quantity of plasmid DNA was steadily released from the pFDNA-CS/PLGA-NPs. An in vitro expression assay indicated that the expression of nanoparticles (NPs) was maintained in the NPs. In an immunization test with specific pathogen-free chickens, the pFDNA-CS/PLGA-NPs induced stronger cellular, humoral, and mucosal immune responses than the plasmid DNA vaccine alone. The pFDNA-CS/PLGA-NPs did not harm 293T cells in an in vitro assay and did not harm chickens in an in vivo assay. Overall, the results indicated that CS-coated PLGA NPs can serve as an efficient and safe mucosal immune delivery system for NDV DNA vaccine.
Keywords: mucosal immune delivery system, immune effect






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